Project

Part:BBa_I3521

Designed by: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)   Group: Antiquity   (2004-01-28)


Test: Quorum threshold level and chemotaxis response, sender cells (I3600.I3520.I3500)

Tests cellular density of asparate-secreting cells needed to induce quorum threshold response. Sender cells are nonmotile and have a trigger controlling the AspA attractant sender and CFP reporter indicating transcription of aspA. Receiver cells have a constitutive promoter controlling YFP reporter. Chemotaxis can be observed by the movement of the YFP-marked receiver cells. Quorum threshold is detected when the LacZ reporter is observed.


Usage and Biology

Chemotaxis response to secreted aspartate (sender cells) testbed, quorum threshold level testbed, quorum sender device


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1222
    Illegal BglII site found at 1671
    Illegal BglII site found at 3136
    Illegal BglII site found at 6455
    Illegal BamHI site found at 5332
    Illegal XhoI site found at 5383
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2563
    Illegal AgeI site found at 2092
    Illegal AgeI site found at 2944
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4073
    Illegal BsaI.rc site found at 5176
    Illegal BsaI.rc site found at 5735
    Illegal SapI site found at 2603


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